ENDONUCLEASE II OF E. coli, I. ISOLATION AND PURIFICATION
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چکیده
منابع مشابه
Isolation and purification of CeqI endonuclease, an isoschizomer of EcoRV.
Ooryrprvcterium equii produces a new restriction endonuclease, an iso-schizemer of EcoKV (1). The bacterial strain does not contain significant levels of other nucleases, so the restriction endonuclease activity can be detected in crude extracts. Ihe enzyme is purified by ammonium sulphate fractionation (50-70 % saturation) and DEAE cellulose chranatography (elutes with 200 mM KC1 in PC buffer)...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1969
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.62.3.934